Seminar talk by Dr. Pavel Srb (IOCB Prague)
Almost everything you wanted to know about biomolecular NMR
The application of modern biomolecular NMR spectroscopy methods on proteins, nucleic acids and their complexes can reveal important molecular properties which are often inaccessible to other biophysical methods. Specifically, the inherent atomic resolution of NMR allows us to localize precisely interaction interfaces or changes in dynamics and molecular conformation. I will briefly explain how structural and dynamical features of molecules can be quickly qualitatively discussed using simple 1D 1 H and 2D N-H correlation spectra. Techniques for studies of interaction of proteins, nucleic acids and their complexes will be demonstrated along with use of spin labels using our recent work on protein-protein and protein-protein-DNA complexes.
