Electrophoresis and Chromatographic Methods (ECHMET group)

The interest of the group of electrophoretic and chromatographic methods – ECHMET – is concentrated on these most widespread separation technics. Basically, we are less interested in the particular results of separations, but instead try to understand separation mechanisms and to propose conditions for obtaining the best separation results. We know a lot about separations as we are able to describe them by mathematical models and solve them using the advanced software we have been developing.

People of the group

Academic Staff

Bohuslav Gaš
prof. RNDr., Ph.D.

Professor

Eva Tesařová
prof. RNDr., CSc.

Professor

Iva Zusková
Doc. RNDr., CSc.,

Scientific secretary, Associate Professor

Květa Kalíková
Doc. RNDr., Ph.D.

Associate professor

Kateřina Ušelová
RNDr., Ph.D.

Assistant professor

Denisa Folprechtová
Mgr.

Doctoral student

Zuzana Kadlecová
Mgr.

Doctoral student

Research Topics

Capillary electrophoresis

  • Theoretical and computer modelling

  • Determination of phys-chem properties of solutes

  • Electrokinetic chromatography and chiral separations

  • Isoelectric focusing

High Performance Liquid Chromatography

  • Chiral separation

  • Method development

  • Characterization of new separation systems

  • New columns testing and evaluation

 

Supercritical Fluid Chromatography

  • Chiral separation

  • Method development

  • Characterization of new separation systems

  • New columns testing and evaluation

Software development

  • User-friendly open-source software tools for CE

  • Simulations of CE and EKC

  • Experimental data evaluation and processing

  • Rationale method optimization

Selected Publications

Z. Vosáhlová, M. Gilar, and K. Kalíková,
Impact of ion-pairing systems choice on diastereomeric selectivity of phosphorothioated oligonucleotides in reversed-phase liquid chromatography
Journal of Chromatography A, 1730, 2024

DOI, BibTeX

D. Folprechtová, P. M. Martínez, and K. Kalíková,
The enantioselective potential of amylose tris(3‑chloro-5-methylphenyl carbamate)-based chiral stationary phase in sub/supercritical fluid and normal phase liquid chromatography
Journal of Chromatography Open, 5, 2024

DOI, BibTeX

Z. Vosáhlová, K. Kalíková, M. Gilar, J. Szymarek, M. Mazurkiewicz-Bełdzińska, and S. Studzińska,
Hydrophilic interaction liquid chromatography with mass spectrometry for the separation and identification of antisense oligonucleotides impurities and nusinersen metabolites
Journal of Chromatography A, 1713, 2024

DOI, BibTeX

Z. Vosahlova-Kadlecova, M. Gilar, K. Molnarova, P. Kozlik, and K. Kalikova,
Mixed-mode column allows simple direct coupling with immobilized enzymatic reactor for on-line protein digestion
Journal of Chromatography B, 1228, 2023
trypsin digestion on-line protein digestion mass spectrometry immobilized enzymatic reactor mixed-mode column

DOI, BibTeX

P. Vankatova, K. Kalikova, and A. Kubickova,
Advantages of polar organic solvent chromatography for enantioseparation of chiral liquid crystals
Journal of Chromatography A, 1709, 2023
chiral resolution liquid crystal enantioseparation liquid chromatography polysaccharide-based chiral selector

DOI, BibTeX

A. Dushkov, Z. Vosahlova, A. Tzintzarov, K. Kalikova, T. Krizek, and I. Ugrinova,
Analysis of the Ibotenic Acid, Muscimol, and Ergosterol Content of an Amanita Muscaria Hydroalcoholic Extract with an Evaluation of Its Cytotoxic Effect against a Panel of Lung Cell Lines In Vitro
Molecules, 28(19), 2023

DOI, BibTeX

B. Gas,
PeakMaster and Simul – Software tools for mastering electrophoresis
TrAC Trends in Analytical Chemistry, 165, 2023

DOI, BibTeX

G. Vigh, and B. Gas,
Evolution of the theoretical description of the isoelectric focusing experiment: III. Carrier ampholyte behavior in transient, bidirectional isotachophoresis
Electrophoresis, 44(78), 2023
capillary isoelectric focusing isoelectric focusing simul 6 simulation transient bidirectional isotachophoresis

DOI, BibTeX

G. Vigh, and B. Gas,
Evolution of the theoretical description of the isoelectric focusing experiment: II. An open system isoelectric focusing experiment is a transient, bidirectional isotachophoretic experiment
Electrophoresis, 44(78), 2023
isoelectric focusing simulation transient bidirectional isotachophoresis

DOI, BibTeX

G. Vigh, and B. Gas,
Evolution of the theoretical description of the isoelectric focusing experiment: I. The path from Svensson's steady-state model to the current two-stage model of isoelectric focusing
ELECTROPHORESIS, 44(78), 2023
capillary isoelectric focusing isoelectric focusing polyacrylamide gel isoelectric focusing steady-state ph gradient two-stage model of isoelectric focusing

DOI, BibTeX

M. Maly, Z. Kadlecova, P. Dubsky, and K. Kalikova,
Semi-empirical description of a retention of small organic probes on AtlantisTM Premier BEH C18 AX mixed-mode column under varying pH and organic modifier conditions
Microchemical Journal, 191, 2023

DOI, BibTeX

Z. Kadlecova, H. Boudova, and K. Kalikova,
The benefits of mixed-mode chromatography columns for separation of peptides and protein digests
Monatshefte fur Chemie - Chemical Monthly, 154(9), 2023

DOI, BibTeX

L. Hasikova J. Zavada T. Serranova P. Kozlik K. Kalikova L. Kotackova J. Trnka D. Zogala et al.
Serum but not cerebrospinal fluid levels of allantoin are increased in de novo Parkinson's disease
npj Parkinson's Disease, 9(1), 2023

DOI, BibTeX

D. Folprechtova, M. G. Schmid, D. W. Armstrong, and K. Kalikova,
The Enantioselective Potential of NicoShell and TeicoShell Columns for Basic Pharmaceuticals and Forensic Drugs in Sub/Supercritical Fluid Chromatography
Molecules, 28(3), 2023

BibTeX

Z. Kadlecová, K. Kalíková, E. Tesařová, and M. Gilar,
Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: effect of temperature
Journal of Chromatography A, 1681, 2022
phosphorothioate oligonucleotides ion-pairing reversed-phase chromatography diastereomeric separation temperature resolution

DOI, BibTeX

P. Vankatova, A. Kubickova, and K. Kalikova,
Enantioseparation of liquid crystals and their utilization as enantiodiscrimination materials
Journal of Chromatography A, 1673, 2022

DOI, BibTeX

Z. Kadlecova, K. Kalikova, E. Tesarova, and M. Gilar,
Phosphorothioate oligonucleotides separation in ion-pairing reversed-phase liquid chromatography: Effect of ion-pairing system
Journal of Chromatography A, 1676, 2022

DOI, BibTeX

K. Kalikova, D. Folprechtova, and Z. Kadlecova,
Sub/superkriticka fluidní chromatografie pro analyzu chiralnich sloucenin
Chemicke Listy, 116, 2022

DOI, BibTeX

S. Abdi, M. Kubů, A. Li, K. Kalíková, and M. Shamzhy,
Addressing confinement effect in alkenes epoxidation using ‘isoreticular’ titanosilicate zeolite catalysts
Catalysis Today (CIS-2021 Young Researchers), 390391, 2022

DOI, BibTeX

L. Hasikova J. Zavada T. Serranova L. Kotackova P. Kozlik K. Kalikova J. Trnka D. Zogala et al.
The effect of tandem coupling of NicoShell and TeicoShell columns in sub/supercritical fluid chromatography on enantioresolution
Parkinsonism & Related Disorders, 90, 2021

DOI, BibTeX

D. Folprechtova, E. Tesarova, and K. Kalikova,
The effect of tandem coupling of NicoShell and TeicoShell columns in sub/supercritical fluid chromatography on enantioresolution
Journal of Separation Science, 44(21), 2021

DOI, BibTeX

Z. Kadlecova, and K. Kalikova,
How ligand and particle types affect retention and separation of structurally diverse biologically active compounds in HPLC
Monatshefte fur Chemie - Chemical Monthly, 152, 2021

DOI, BibTeX

D. Vrbata, S. Kereiche, K. Kalíková, and M. Uchman,
Stimuli-responsive multifunctional micelles of ABC vs. ACB triblock terpolymers using reversible covalent bonding of phenylboronic acid: controlled synthesis, self-assembly and model drug release
Journal of Molecular Liquids, 335(1), 116528, 2021

DOI, BibTeX

D. Folprechtova, and K. Kalikova,
Macrocyclic glycopeptide-based chiral selectors for enantioseparation in sub/supercritical fluid chromatography
Analytical Science Advances, 2(12), 2021

DOI, BibTeX

P. Kozlik, J. Vaclova, and K. Kalikova,
Mixed-mode hydrophilic interaction/ion-exchange liquid chromatography - Separation potential in peptide analysis
Microchemical Journal, 165, 2021

DOI, BibTeX

Z. Kadlecova, P. Kozlik, E. Tesarova, M. Gilar, and K. Kalikova,
Characterization and comparison of mixed-mode and reversed-phase columns; interaction abilities and applicability for peptide separation
Journal of Chromatography A, 1648, 2021

DOI, BibTeX

S. Abdi, M. Kubu, A. Li, K. Kalikova, and M. Shamzhy,
Addressing confinement effect in alkenes epoxidation using "isoreticular" titanosilicate zeolite catalysts
Catalysis Today, 2021

DOI, BibTeX

P. Vankatova, A. Kubickova, and K. Kalikova,
How mobile phase composition and column temperature affect enantiomer elution order of liquid crystals on amylose tris(3-chloro-5-methylphenylcarbamate) as chiral selector
Electrophoresis, 42(1718), 2021

DOI, BibTeX

D. Folprechtova, K. Kalikova, K. Kadkhodaei, C. Reiterer, D. W. Armstrong, E. Tesarova, and M. G. Schmid,
Enantioseparation performance of superficially porous particle vancomycin-based chiral stationary phases in supercritical fluid chromatography and high performance liquid chromatography; applicability for psychoactive substances
Journal of Chromatography A, 1637, 2021

DOI, BibTeX

T. Cadart D. Necas R. P. Kaiser L. Favereau I. Cisarova R. Gyepes J. Hodacova K. Kalikova et al.
Rhodium-Catalyzed Enantioselective Synthesis of Highly Fluorescent and CPL-Active Dispiroindeno[2,1-c]fluorenes
Chemistry - A European Journal, 27(44), 2021

DOI, BibTeX

B. Gas, and P. Bravenec,
Simul 6: A fast dynamic simulator of electromigration
Electrophoresis, 42(1213), 2021

DOI, BibTeX

T. Novotny, and B. Gas,
Mathematical model of electromigration allowing the deviation from electroneutrality
Electrophoresis, 42(78), 2021

DOI, BibTeX

R. Lunkad, A. Murmiliuk, P. Hebbeker, M. Boublík, Z. Tošner, M. Štěpánek, and P. Košovan,
Quantitative prediction of charge regulation in oligopeptides
Molecular Systems Design & Engineering, 6, 122-131, 2020

DOI, BibTeX

Z. Kadlecová, K. Kalíková, M. Ansorge, M. Gilar, and E. Tesařová,
The effect of particle and ligand types on retention and peak shape in liquid chromatography
Microchemical Journal, 159, 2020

DOI, BibTeX

D. Folprechtová, and K. Kalíková,
Macrocyclic glycopeptide-based chiral selectors for enantioseparation in sub/supercritical fluid chromatography
Analytical Science Advances, 2(12), 2020

DOI, BibTeX

T. Novotný, and B. Gaš,
Mathematical model of electromigration allowing the deviation from electroneutrality
Electrophoresis, 42(78), 2020

DOI, BibTeX

D. Aldhayan, K. Kalíková, M. R. Shaik, M. R. H. Siddiqui, and J. Přech,
Selective Oxidation of Citronellol over Titanosilicate Catalysts
Catalysts, 10(11), 2020

DOI, BibTeX

P. Vaňkátová, T. Šrolerová, A. Kubíčková, and K. Kalíková,
Fast UHPLC enantioseparation of liquid crystalline materials with chiral center based on octanol in reversed-phase and polar organic mode
Monatshefte fur Chemie- Chemical Monthly, 151(8), 2020

DOI, BibTeX

M. Ansorge, B. Gaš, M. Boublík, M. Malý, J. Šteflová, V. Hruška, and G. Vigh,
CE determination of the thermodynamic p K a values and limiting ionic mobilities of 14 low molecular mass UV absorbing ampholytes for accurate characterization of the pH gradient in carrier ampholytes-based IEF and its numeric simulation
Electrophoresis, 41(78), 2020

DOI, BibTeX

T. Novotný, and B. Gaš,
Electrolysis phenomena in electrophoresis
Electrophoresis, 41(78), 2020

DOI, BibTeX

M. Dvořák, M. Kalus, M. Dovhunová, P. Dubský, and B. Gaš,
The dynamics of band (peak) shape development in capillary zone electrophoresis in the case of two co-migrating analytes: The displacement and the tag-along effects
Electrophoresis, 41(78), 2020

DOI, BibTeX

M. Urbańska, P. Vaňkátová, A. Kubíčková, and K. Kalíková,
Synthesis, characterisation and supercritical fluid chromatography enantioseparation of new liquid crystalline materials
Liquid Crystals, 47(12), 2020

DOI, BibTeX

D. Folprechtová, O. Kozlov, D. W. Armstrong, M. G. Schmid, K. Kalíková, and E. Tesařová,
Enantioselective potential of teicoplanin- and vancomycin-based superficially porous particles-packed columns for supercritical fluid chromatography
Journal of Chromatography A, 1612, 2020

DOI, BibTeX

P. Kozlik, L. Hasikova, B. Stiburkova, J. Zavada, and K. Kalikova,
Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress
Analytical Biochemistry, 589, 2020

DOI, BibTeX

M. Dovhunová, M. Malý, P. Dubský, G. S. Gerlero, and P. A. Kler,
Generalized model of the linear theory of electromigration and its application to electrokinetic chromatography: Capillary zone electrophoretic systems with complex-forming equilibria
Journal of Chromatography A, 1610, 2020

DOI, BibTeX

M. Boublík, M. Riesová, V. Hruška, and J. Šteflová,
Online preconcentration of weak electrolytes at the pH boundary induced by a system zone in capillary zone electrophoresis
Analytica Chimica Acta, 1085, 126-135, 2019

DOI, BibTeX

O. Kozlov, Z. Kadlecová, E. Tesařová, and K. Kalíková,
Evaluation of separation properties of stationary phases in supercritical fluid chromatography\mathsemicolon deazapurine nucleosides case study
Microchemical Journal, 150, 104137, 2019

DOI, BibTeX

O. S. Ahmed, M. Malý, Y. Ladner, L. Philibert, P. Dubský, and C. Perrin,
Influence of salt and acetonitrile on the capillary zone electrophoresis analysis of imatinib in plasma samples
Electrophoresis, 40(21), 2810-2819, 2019

DOI, BibTeX

L. A. Papp, G. Hancu, Á. Gyéresi, H. Kelemen, Z.-I. Szabó, B. Noszál, P. Dubský, and G. Tóth,
Chiral separation of lansoprazole and rabeprazole by capillary electrophoresis using dual cyclodextrin systems
Electrophoresis, 40(21), 2799-2805, 2019

DOI, BibTeX

L. Hasikova M. Pavlikova H. Hulejova P. Kozlik K. Kalikova A. Mahajan M. Herrmann B. Stiburkova et al.
Serum uric acid increases in patients with systemic autoimmune rheumatic diseases after 3~months of treatment with TNF inhibitors
Rheumatology International, 39(10), 1749-1757, 2019

DOI, BibTeX

O. Kozlov, K. Kalíková, T. Gondová, M. Budovská, A. Salayová, and E. Tesařová,
Fast enantioseparation of indole phytoalexins in additive free supercritical fluid chromatography
Journal of Chromatography A, 1596, 209-216, 2019

DOI, BibTeX

P. Vaňkátová, A. Kubíčková, M. Cigl, and K. Kalíková,
Ultra-performance chromatographic methods for enantioseparation of liquid crystals based on lactic acid
The Journal of Supercritical Fluids, 146, 217-225, 2019

DOI, BibTeX

O. Kozlov, Z. Kadlecová, M. Gilar, T. Gondová, K. Kalíková, and E. Tesařová,
Systematic evaluation of selected supercritical fluid chromatography diol- and diethylamine-based columns for application in hydrophilic interaction liquid chromatography
Separation Science Plus, 2(3), 81-88, 2019

DOI, BibTeX

D. Folprechtová, K. Kalíková, P. Kozlík, and E. Tesařová,
The degree of substitution affects the enantioselectivity of sulfobutylether-pbeta-cyclodextrin chiral stationary phases
Electrophoresis, 40(15), 1972-1977, 2019

DOI, BibTeX

N. Ouadah, C. Moire, F. Brothier, J.-F. Kuntz, M. Malý, P. Dubský, and H. Cottet,
Mobility Shift Affinity Capillary Electrophoresis at High Ligand Concentrations: Application to Aluminum Chlorohydrate\textendashProtein Interactions
ACS Omega, 3(12), 17547-17554, 2018

DOI, BibTeX

M. Malý, M. Dovhunová, M. Dvořák, G. S. Gerlero, P. A. Kler, V. Hruška, and P. Dubský,
Generalized Model of the Linear Theory of Electromigration and its Application to Electrokinetic Chromatography: Theory and Software PeakMaster 6 \textendash Next Generation
Electrophoresis, 40(5), 683-692, 2018

DOI, BibTeX

A. C. Bidulock, P. Dubský, A. den Berg, and J. C. Eijkel,
Integrated internal standards: A sample prep-free method for better precision in microchip CE
Electrophoresis, 40(5), 756-765, 2018

DOI, BibTeX

M. Dvořák, P. Dubský, M. Dovhunová, and B. Gaš,
The dynamics of band (peak) shape development in capillary zone electrophoresis in light of the linear theory of electromigration
Electrophoresis, 40(5), 668-682, 2018

DOI, BibTeX

P. Vaňkátová, K. Kalíková, and A. Kubíčková,
Ultra-performance supercritical fluid chromatography: A powerful tool for the enantioseparation of thermotropic fluorinated liquid crystals
Analytica Chimica Acta, 1038, 191-197, 2018

DOI, BibTeX